Offensive Positions
| POS | Starter | Second | Third+ |
|---|---|---|---|
| Quarterback | J. GoffJared Goff | N. SudfeldNate Sudfeld | — |
| Running Back | J. WilliamsJamaal Williams | D. SwiftD'Andre Swift | |
| Fullback | J. CabindaJason Cabinda | — | — |
| Wide Receiver | A. St. BrownAmon-Ra St. Brown | D. CharkD.J. Chark | |
| Tight End | B. WrightBrock Wright | S. ZylstraShane Zylstra | |
| Left Tackle | T. DeckerTaylor Decker | D. SkipperDan Skipper | |
| Left Guard | J. JacksonJonah Jackson | L. StenbergLogan Stenberg | — |
| Center | F. RagnowFrank Ragnow | R. PierschbacherRoss Pierschbacher | — |
| Right Guard | E. BrownEvan Brown | K. AwosikaKayode Awosika | — |
| Right Tackle | P. SewellPenei Sewell | M. NelsonMatt Nelson |
Defensive Positions
| POS | Starter | Second | Third+ |
|---|---|---|---|
| Left Defensive End | R. OkwaraRomeo Okwara | J. PaschalJosh Paschal | |
| Right Defensive End | A. HutchinsonAidan Hutchinson | J. CominskyJohn Cominsky | |
| Left Defensive Tackle | A. McNeillAlim McNeill | B. JonesBenito Jones | — |
| Right Defensive Tackle | I. BuggsIsaiah Buggs | M. BrockersMichael Brockers | |
| Strongside Linebacker | J. HoustonJames Houston | D. BarnesDerrick Barnes | |
| Middle Linebacker | A. AnzaloneAlex Anzalone | J. WoodsJosh Woods | — |
| Weakside Linebacker | M. RodriguezMalcolm Rodriguez | C. BoardChris Board | — |
| Right Cornerback | J. OkudahJeff Okudah | A. OruwariyeAmani Oruwariye | |
| Left Cornerback | J. JacobsJerry Jacobs | W. HarrisWill Harris | |
| Strong Safety | K. JosephKerby Joseph | I. MelifonwuIfeatu Melifonwu | |
| Free Safety | D. ElliottDeShon Elliott | C. MooreC.J. Moore |
Special Teams Positions
| POS | Starter | Second | Third+ |
|---|---|---|---|
| Punter | J. FoxJack Fox | — | — |
| Kicker | M. BadgleyMike Badgley | — | — |
| Long Snapper | S. DalyScott Daly | — | — |
| Holder | J. FoxJack Fox | — | — |
| Punt Returner | K. RaymondKalif Raymond | A. St. BrownAmon-Ra St. Brown | — |
| Kick Returner | J. JacksonJustin Jackson | — | — |